Vitamin D3 (25 Hydroxy) test is a blood test that measures the level of vitamin D in the body. Vitamin D is a vital nutrient that helps the body absorb calcium and maintain strong bones. It also plays a crucial role in regulating the immune system, promoting cell growth, and reducing inflammation. The 25-hydroxyvitamin D test is the most accurate way to measure vitamin D levels in the body because it reflects the amount of vitamin D that is stored in the body. Low levels of vitamin D have been linked to a range of health issues, including bone and muscle weakness, increased risk of certain cancers, and autoimmune disorders. A doctor may recommend a vitamin D3 test if they suspect a person may be deficient in vitamin D or if they have symptoms that may be related to a vitamin D deficiency.