The protein total test is a blood test that measures the total amount of protein in the blood, including albumin and globulin. Albumin is a protein that is made by the liver and helps to transport hormones, drugs, and other substances throughout the body. Globulin is a group of proteins that includes antibodies, which are important for fighting infections, as well as other proteins that transport hormones and other substances. The A:G ratio, which is the ratio of albumin to globulin, can also be calculated from the test results. This ratio can be used to help diagnose certain medical conditions, such as liver disease or kidney disease, and to monitor the effectiveness of treatment.